Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.
2019
Authors:
Mosić, MirjanaTrifković, Jelena
Vovk, Irena
Gašić, Uroš
Tešić, Živoslav
Šikoparija, Branko
Milojković-Opsenica, Dušanka
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.
Keywords:
HPTLC; UHPLC-MS/MS; antioxidant activity; bee-pollen; phenolic profileSource:
Biomolecules, 2019, 9, 12, 783-Funding / projects:
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
- Slovenian Research Agency (research core funding No. P1-0005)
- RegPot project FCUB ERA GA No. 256716
DOI: 10.3390/biom9120783
PubMed: 31779186