Mogućnost masovne proizvodnje različitih kultivara hrizantema (chrysanthemum morifolium) primenom in vitro kulture
Mass production of different chrysanthemum (chrysanthemum morifoliiun) cultivars by culture in vitro
Abstract:
in vitro plant regeneration of 19 chrysanthemum cultivars: cvs. "Violet Spider" (VS), "White Spider" (WS), "Yellow Spider" (YS), "Vesuvio" (V) "Copper Spider" (CS), "Aibert Heyn" (AH), "Papagaj" (P), "Reagan White" (RW), "Reagan Sunny" (RS), "Reagan Splendid" (RSp), "June Yellow" (JY) "June Rose" (JR), Tigerrag" (T), "Yellow Jubilee" (YJ), "Bronz Mundial" (BM), "Fanshine Improved" (FI), "Klondike" (K), "Pink Snowdon" (PS) and "Rivarly" (R) was investigated. Induction of morphogenesis is achieved in steam segment culture on MS medium. Shoot multiplication was investigated on six MS media supplemented with BAP, kinetine or TDZ (1 mgL1, respectively) and NAA (0.1 or 0.5mgL-1 respectively). The best shoot multiplication index were observed on MS media supplemented with BAP as cytokinin for ten cultivars. According to these results, the annual plant production could be 1.4 x 1013 shoots for cv. "R", and the lowest (7.8 x 106 shoots) for cv. "RSp". Rooting of shoots (95-100 %) was satisfactory on MS hormone free medium. Potted chrysanthemum plants were acclimatised in arrange 55-84%.