Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach
2019
Authors:
Dragićević, MilanĆuković, Katarina
Zdravković-Korać, Snežana
Simonović, Ana
Bogdanović, Milica
Todorović, Slađana
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.
Keywords:
Germination; Glutamine synthetase; Grain filling; Knockout mutant; PhenotypeSource:
Archives of Biological Sciences, 2019, 71, 3, 443-453Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
DOI: 10.2298/ABS190315026D
ISSN: 0354-4664
WoS: 000491998600007
Scopus: 2-s2.0-85076490995
URI
http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900026Dhttp://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4099
https://radar.ibiss.bg.ac.rs/handle/123456789/3568