Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.
2020
Authors:
Pantić, IgorJeremić, Rada
Dacić, Sanja
Peković, Sanja
Pantić, Senka
Đelić, Marina
Vitić, Zagorka
Brkić, Predrag
Brodski, Claude
Document Type:
Article (Accepted Version)
,
© Microscopy Society of America 2020.
Metadata
Show full item recordAbstract:
Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.
Keywords:
Dentate gyrus; Gray-level co-occurrence matrix analysis; Hippocampus; Sensorimotor cortex ablation; Texture; Traumatic brain injurySource:
Microscopy and Microanalysis, 2020, 26, 1, 166-172Funding / projects:
- Cellular and molecular basis of neuroinflamation: potential targets for translational medicine and therapy (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
- Light microscopy, electron microscopy, immunomorphologic, molecular biology and genetic investigations of malignant and nonmalignant renal diseases. (RS-MESTD-Basic Research (BR or ON)-175059)
- Cellular and molecular basis of malignant and cardiovascular diseases-clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- project 92018 of the Mediterranean Society for Metabolic Syndrome, Diabetes and Hypertension in Pregnancy DEGU
DOI: 10.1017/S143192762000001X
ISSN: 1431-9276
PubMed: 31948501