Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.
2020
Autori:
Pantić, IgorJeremić, Rada
Dacić, Sanja
Peković, Sanja
Pantić, Senka
Đelić, Marina
Vitić, Zagorka
Brkić, Predrag
Brodski, Claude
Tip dokumenta:
Članak u časopisu (Recenzirana verzija)
,
© Microscopy Society of America 2020.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.
Ključne reči:
Dentate gyrus; Gray-level co-occurrence matrix analysis; Hippocampus; Sensorimotor cortex ablation; Texture; Traumatic brain injuryIzvor:
Microscopy and Microanalysis, 2020, 26, 1, 166-172Finansiranje / projekti:
- Ćelijska i molekulska osnova neuroinflamacije: potencijala ciljna mesta za translacionu medicinu i terapiju (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
- Optičko mikroskopska, imunomorfološka, molekularno-biološka i genetska ispitivanja malignih i nemalignih bolesti bubrega (RS-MESTD-Basic Research (BR or ON)-175059)
- Ćelijske i molekulske osnove malignih i kardiovaskularnih oboljenja-kliničke implikacije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- project 92018 of the Mediterranean Society for Metabolic Syndrome, Diabetes and Hypertension in Pregnancy DEGU
DOI: 10.1017/S143192762000001X
ISSN: 1431-9276
PubMed: 31948501