HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
2019
Autori:
Stanković, JovanaNovaković, Miroslav
Tešević, Vele
Ćirić, Ana
Soković, Marina
Zdunić, Gordana
Dajić-Stevanović, Zora
Gođevac, Dejan
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
This study was performed to determine the main antibacterial compounds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.
Ključne reči:
Antimicrobial activity; Direct bioautography; Antibiofilm; Antiquorum potentialIzvor:
Journal of the Serbian Chemical Society, 2019, 84, 12, 1355-1365Finansiranje / projekti:
- Bioaktivni prirodni proizvodi samoniklih, gajenih i jestivih biljaka: određivanje struktura i aktivnosti (RS-MESTD-Basic Research (BR or ON)-172053)
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
- Morfološka, hemijska, farmakološka i agronomska karakterizacija panonskog timijana (Thymus pannonicus All., Lamiaceae), sa ciljem njegove održive proizvodnje u intenzivnom sistemu ratarenja (RS-MESTD-Technological Development (TD or TR)-31089)
DOI: 10.2298/jsc190513106s
ISSN: 0352-5139