A perspective view on the nanomotion detection of living organisms and its features
2020
Authors:
Venturelli, LeonardoKohler, Anne-Céline
Stupar, Petar
Villalba, Maria I.
Kalauzi, Aleksandar
Radotić, Ksenija
Bertacchi, Massimiliano
Dinarelli, Simone
Girasole, Marco
Pešić, Milica
Banković, Jasna
Vela, Maria E.
Yantorno, Osvaldo
Willaert, Ronnie
Dietler, Giovanni
Longo, Giovanni
Kasas, Sandor
Document Type:
Article (Accepted Version)
,
© 2020 John Wiley & Sons Ltd
Metadata
Show full item recordAbstract:
The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
Keywords:
AFM; Antibiotic susceptibility test; Cellular metabolism; Nanobiosensors; Nanomechanical sensors; Nanomotion; Single cell investigationSource:
Journal of Molecular Recognition, 2020, e2849-Funding / projects:
- Identification of predictive molecular markers for cancer progression, response to therapy and disease outcome (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41031)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
- Agencia Nacional de Promoción Científica y Tecnológica. Grant Numbers: PICT 2016‐0679, PICT 2017‐2444, PUE 22920170100100CO
- European Space Agency. Grant Number: PRODEX
- Federaal Wetenschapsbeleid
- Gebert Rüf Stiftung. Grant Number: GRS‐024/14
- Ministero della Salute. Grant Number: GR‐2009‐1605007
- NASA Exoplanet Science Institute. Grant Number: NNH16ZDA001N‐CLDTCH
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung. Grant Numbers: 200021‐144321, 407240‐167137, CRSII5_173863
DOI: 10.1002/jmr.2849
ISSN: 0952-3499
PubMed: 32227521