Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L.
A rapid protocol for DNA extraction and a comparison of tree protocols for RNA extraction from Impatiens walleriana L.
dc.creator | Milošević, Snežana | |
dc.creator | Cingel, Aleksandar | |
dc.creator | Ninković, Slavica | |
dc.creator | Nikolić, Dragana | |
dc.creator | Jevremović, Slađana | |
dc.creator | Tadić, Vojin | |
dc.creator | Subotić, Angelina | |
dc.date.accessioned | 2015-08-28T10:26:51Z | |
dc.date.available | 2015-08-28T10:26:51Z | |
dc.date.issued | 2011 | sr |
dc.date.issued | 2011 | |
dc.identifier.issn | 0354-5695 | sr |
dc.identifier.other | Rad_konverzija_418 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/365 | |
dc.description.abstract | In order to detect and identify viruses in the plant material or to confirm the expression of a gene after genetic transformation it is necessary to have a fast, simple and reliable method for the extraction of DNA and RNA. In this study, we tested a protocol for DNA and three protocols for RNA extraction from plants of I. walleriana L. RNA obtained by different extraction methods significantly differed in its yield and purity. The optimum method proved to be the one applicable to plant species that contain large amounts of secondary metabolites (polyphenols and polysaccharides). Nucleic acid extracted by the application of these methods showed satisfactory results in PCR and RT-PCR analysis. | en |
dc.description.abstract | Za detekciju i identifikaciju virusa u biljnom materijalu ili potvrdu ekspresije nekog gena nakon genetičkih transformacija neophodno je imati brzu, jednostavnu i pouzdanu metodu za ekstrakciju DNK i RNK. U ovom radu smo testirali jedan protokol za izolaciju DNK i tri za ekstrakciju RNK iz biljaka Impatiens walleriana L. RNK dobijena primenom različitih metoda za ekstrakciju znatno se razlikovala po prinosu i čistoći. Kao optimalna metoda se pokazala ona koja se primenjuje za biljne vrste koje sadrže velike količine sekundarnih metabolita (polifenola i polisaharida). Nukleinske kiseline ekstrahovane primenom ovih metoda pokazale su zadovoljavajuće rezultate u PCR i RT-PCR analizama. | sr |
dc.description.sponsorship | Projekat ministarstva br. TR 31019 | sr |
dc.language.iso | srp | sr |
dc.rights | openAccess | sr |
dc.source | Journal of Scientific Agricultural Research | sr |
dc.subject | CTAB | SRP |
dc.subject | DNK | SRP |
dc.subject | fenoli | SRP |
dc.subject | LiCl | SRP |
dc.subject | precipitacija RNK | SRP |
dc.subject | TRIzol | SRP |
dc.title | Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L. | sr |
dc.title | A rapid protocol for DNA extraction and a comparison of tree protocols for RNA extraction from Impatiens walleriana L. | en |
dc.type | article | sr |
dc.rights.license | ARR | |
dcterms.abstract | Цингел, Aлександар Д.; Јевремовић, Слађана; Милошевић, Снежана; Суботић, Aнгелина; Тадић, Војин; Нинковић, Славица; Николић, Драгана; | |
dc.citation.issue | 1 | sr |
dc.citation.volume | 72 | sr |
dc.citation.spage | 15 | sr |
dc.citation.epage | 21 | sr |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M52 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_365 |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |