Population diversity of n-alkanes in the needle cuticular wax of relicts pinus heldreichii and P. peuce from the scardo-pindic mountains
2020
Authors:
Nikolić, BiljanaTešević, Vele
Đorđević, Iris
Todosijević, Marina
Mitić, Zorica
Bojović, Srđan
Marin, Petar D.
Document Type:
Article (Published version)
,
© 2020, 2020 Biljana M. Nikolić, Iris Đorđević, Marina Todosijević, Zorica Mitić, Srdjan Bojović, Petar D. Marin
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Show full item recordAbstract:
The variability of n-alkanes in the needle cuticular wax of Pinus heldreichii and P. peuce in two natural populations from the Scardo-Pindic mountains was investigated for the first time. Gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) analyses of two-year-old needles were performed us-ing an Agilent 7890A GC equipped with an inert 5975C XL EI/CI mass spectrometer detector (MSD) and a flame ionization detector (FID) connected by a capillary flow technology 2-way splitter with make-up. An HP-5MS capillary column was used. n-Alkanes ranged from C19 to C33. In P. heldreichii the most abundant were C23, C25 and C27 (16.3, 15.6 and 12.8 % on average, respectively), while in P. peuce they were C29, C25, and C27 (16.5, 15.3 and 13.5 % on average, resp.). Mid-chain and long-chain n-alkanes prevailed in both species. Principle component analysis (PCA) and Cluster analyses of five and six n-alkanes, respectively, showed divergence of the Scardo-Pindic populations from the Dinaric ones.
Keywords:
Cluster analysis; Diversity; N-alkanes; P. peuce; Pinus heldreichii; Principle component analysis (PCA)Source:
Macedonian Journal of Chemistry and Chemical Engineering, 2020, 39, 1, 41-48Funding / projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-MESTD-Basic Research (BR or ON)-173029)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
- Evaluation of ecophysiological and genetic plant diversity in forest ecosystems (RS-MESTD-Basic Research (BR or ON)-173011)
DOI: 10.20450/mjcce.2020.1951
ISSN: 1857-5552