Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
2020
Autori:
Cvetić Antić, TijanaJanošević, Dušica
Maksimović, Vuk M.
Živić, Miroslav
Budimir, Snežana
Glamočlija, Jasmina
Mitrović, Aleksandra Lj
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2019 Elsevier GmbH.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
Ključne reči:
Fungal leaf infection; Fusaric acid; Plant antioxidative system; Stomata; Transition from biotrophy to necrotrophyIzvor:
Journal of Plant Physiology, 2020, 244, 153086-Finansiranje / projekti:
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-MESTD-Basic Research (BR or ON)-173040)
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
DOI: 10.1016/j.jplph.2019.153086
ISSN: 1618-1328
PubMed: 31812905