Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.
2020
Authors:
Stojković, DejanDias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena
C F R Ferreira, Isabel
Soković, Marina
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
Keywords:
O. spinosa; Aerial parts; Antibiofilm; Antifungal; Cytotoxicity; Methanolic extract; Phenolic compoundsSource:
Pharmaceuticals (Basel, Switzerland), 2020, 13, 4, 78-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- TRANSCoLAB 0612_TRANS_CO_LAB_2_P
- 0377_Iberphenol_6_E
- UIDB/00690/2020
DOI: 10.3390/ph13040078
ISSN: 1424-8247
PubMed: 32344670
WoS: 000534047200014
Scopus: 2-s2.0-85089723909
URI
https://www.mdpi.com/1424-8247/13/4/78http://www.ncbi.nlm.nih.gov/pubmed/32344670
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7243113
https://radar.ibiss.bg.ac.rs/123456789/3854