Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells.
2020
Autori:
Stojković, DejanDias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena
C F R Ferreira, Isabel
Soković, Marina
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
Ključne reči:
O. spinosa; Aerial parts; Antibiofilm; Antifungal; Cytotoxicity; Methanolic extract; Phenolic compoundsIzvor:
Pharmaceuticals (Basel, Switzerland), 2020, 13, 4, 78-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- TRANSCoLAB 0612_TRANS_CO_LAB_2_P
- 0377_Iberphenol_6_E
- UIDB/00690/2020
DOI: 10.3390/ph13040078
ISSN: 1424-8247
PubMed: 32344670
WoS: 000534047200014
Scopus: 2-s2.0-85089723909
URI
https://www.mdpi.com/1424-8247/13/4/78http://www.ncbi.nlm.nih.gov/pubmed/32344670
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7243113
https://radar.ibiss.bg.ac.rs/123456789/3854