Chemo-protective and regenerative effects of diarylheptanoids from the bark of black alder (Alnus glutinosa) in human normal keratinocytes
2015
Authors:
Dinić, JelenaRanđelović, Teodora
Stanković, Tijana
Dragoj, Miodrag
Isaković, Aleksandra
Novaković, Miroslav
Pešić, Milica
Document Type:
Article (Published version)
,
© 2015 Elsevier Inc.
Metadata
Show full item recordAbstract:
Medicinal plants are recognized from ancient times as a source of diverse therapeutic agents and many of them are used as dietary supplements. Comprehensive approaches are needed that would identify bioactive components with evident activity against specific indications and provide a better link between science (ethno-botany, chemistry, biology and pharmacology) and market. Recently, the bark of black alder (Alnus glutinosa) appeared at market in the form of food supplement for treatment of different skin conditions. This study aimed to evaluate protective effects of two diarylheptanoids isolated from the bark of black alder: platyphylloside, 5(S)-1,7-di(4-hydroxyphenyl)-3-heptanone-5-O-β-D-glucopyranoside (1) and its newly discovered analog 5(S)-1,7-di(4-hydroxyphenyl)-5-O-β-D-[6-(E-p-coumaroylglucopyranosyl)]heptane-3-one (2) towards doxorubicin damaging activity. To that end, we employed HaCaT cells, non-cancerous human keratinocytes commonly used for skin regenerative studies. Diarylheptanoids significantly antagonized the effects of doxorubicin by lowering the sensitivity of HaCaT cells to this drug. Compound 2 prevented doxorubicin-induced cell death by activating autophagy. Both 1 and 2 protected HaCaT cells against doxorubicin-induced DNA damage. They significantly promoted migration and affected F-actin distribution. These results indicate that chemo-protective effects of diarylheptanoids may occur at multiple subcellular levels. Therefore, diarylheptanoids 1 and 2 could be considered as protective agents for non-cancerous dividing cells during chemotherapy.
Note:
Related to: https://radar.ibiss.bg.ac.rs/123456789/3864.
Keywords:
Diarylheptanoid; Curcumin; Doxorubicin; DNA damage; Cell motility; ChemoprotectionSource:
Fitoterapia, 2015, 105, 169-176Funding / projects:
- Identification of predictive molecular markers for cancer progression, response to therapy and disease outcome (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41031)
DOI: 10.1016/j.fitote.2015.07.003
ISSN: 0367-326X
PubMed: 26162555
WoS: 000361401500027
Scopus: 2-s2.0-84937539499
URI
https://www.sciencedirect.com/science/article/abs/pii/S0367326X1530040Xhttps://radar.ibiss.bg.ac.rs/123456789/3865