Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)
2016
Authors:
Nenadić, MarijaSoković, Marina
Glamočlija, Jasmina
Ćirić, Ana
Perić Mataruga, Vesna
Tešević, Vele
Vujisić, Ljubodrag
Todosijević, Marina
Vesović, Nikola
Ćurčić, Srećko
Document Type:
Article (Published version)
,
© Cambridge University Press 2016
Metadata
Show full item recordAbstract:
The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.
Keywords:
Laemostenus (Pristonychus) punctatus; pygidial gland secretion; antibacterial and antifungal activity; human pathogens; microdilution; morphologySource:
Bulletin of Entomological Research, 2016, 106, 4, 474-480Funding / projects:
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- The role of autophagy in regulation of cancer cell death (RS-MESTD-Basic Research (BR or ON)-173053)
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
- Ontogenetic characterization of phylogenetic biodiversity (RS-MESTD-Basic Research (BR or ON)-173038)
DOI: 10.1017/S0007485316000109
ISSN: 0007-4853
PubMed: 27018928
WoS: 000379418000006
Scopus: 2-s2.0-84961684194
URI
https://www.cambridge.org/core/journals/bulletin-of-entomological-research/article/antimicrobial-activity-of-the-pygidial-gland-secretion-of-the-troglophilic-ground-beetle-laemostenus-pristonychus-punctatus-dejean-1828-insecta-coleoptera-carabidae/8DF8DE1725619065F0C0C1631B5CF359https://radar.ibiss.bg.ac.rs/handle/123456789/3970