Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)
2016
Authors:
Nenadić, MarijaSoković, Marina
Glamočlija, Jasmina
Ćirić, Ana
Perić Mataruga, Vesna
Ilijin, Larisa
Tešević, Vele
Vujisić, Ljubodrag
Todosijević, Marina
Vesović, Nikola
Ćurčić, Srećko
Document Type:
Article (Published version)
,
© Springer-Verlag Berlin Heidelberg 2016
Metadata
Show full item recordAbstract:
The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.
Keywords:
Ground beetles; Pygidial gland secretion; Antibacterial and antifungal activity; Human pathogens; Microdilution; Morpho-histologySource:
The Science of Nature, 2016, 103, 3-4, 34-Funding / projects:
- Ontogenetic characterization of phylogenetic biodiversity (RS-MESTD-Basic Research (BR or ON)-173038)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.1007/s00114-016-1358-z
ISSN: 0028-1042
PubMed: 27000261
WoS: 000372752800019
Scopus: 2-s2.0-85006979878
URI
https://link.springer.com/article/10.1007/s00114-016-1358-zhttps://pubmed.ncbi.nlm.nih.gov/27000261/
https://radar.ibiss.bg.ac.rs/handle/123456789/3981