Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety
2021
Authors:
Milinčić, Danijel D.Stanisavljević, Nemanja S.
Kostić, Aleksandar
Soković Bajić, Svetlana
Kojić, Milan O.
Gašić, Uroš
Barać, Miroljub B.
Stanojević, Sladjana P.
Tešić, Živoslav Lj.
Pešić, Mirjana B.
Document Type:
Article (Published version)
,
© 2020 Elsevier Ltd.
Metadata
Show full item recordAbstract:
Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS•+ DPPH• and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.
Keywords:
Antioxidant activity; Cellular antioxidant activity; Cytotoxic activity; Phenolic compounds; Wine by-productsSource:
LWT, 2021, 138, 110739-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.1016/j.lwt.2020.110739
ISSN: 0023-6438