Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen.
2021
Authors:
Kostić, Aleksandar Ž.Milinčić, Danijel D.
Stanisavljević, Nemanja S.
Gašić, Uroš
Lević, Steva
Kojić, Milan O.
Lj Tešić, Živoslav
Nedović, Viktor
Barać, Miroljub B.
Pešić, Mirjana B.
Document Type:
Article (Published version)
,
© 2021 Elsevier Ltd.
Metadata
Show full item recordAbstract:
The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7-76.2%) followed by phenolic acids (14.2-17.4%). Among individual compounds quercetin-3-O-glucoside (155.1-197.2 μg/L) and p-coumaric acid (29.5-30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
Keywords:
Antioxidant properties; Goat milk/pollen powder; In vitro polyphenol bioaccessibility; Monofloral sunflower bee-collected pollenSource:
Food Chemistry, 2021, 351, 129310-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
DOI: 10.1016/j.foodchem.2021.129310
ISSN: 0308-8146
PubMed: 33640767
WoS: 000632300600001
Scopus: 2-s2.0-85101327994
URI
https://linkinghub.elsevier.com/retrieve/pii/S0308814621003162http://www.ncbi.nlm.nih.gov/pubmed/33640767
https://radar.ibiss.bg.ac.rs/handle/123456789/4158