Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies.
2021
Autori:
Lazarević, MilicaNikolovski, Neda
Stanisavljević, Suzana
Dimitrijević, Mirjana
Stegnjaić, Goran
Krishnamoorthy, Gurumoorthy
Mostarica Stojković, Marija
Miljković, Đorđe
Jevtić, Bojan
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2021 Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8+CD11bc+MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.
Ključne reči:
CD4(+) T cells; CD8(+) macrophages; CFA; EAEIzvor:
Journal of Neuroimmunology, 2021, 354, 577547-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- European Research Council starting grant (GAMES; 635617)
- German research foundation (DFG) SFB TR-128 (Project A1)
- Max Planck Society
Povezane informacije:
- Povezani sadržaj
https://radar.ibiss.bg.ac.rs/handle/123456789/4200
DOI: 10.1016/j.jneuroim.2021.577547
ISSN: 0165-5728
PubMed: 33765502