Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities
2021
Authors:
Veličković, IvonaŽižak, Željko
Rajčević, Nemanja
Ivanov, Marija
Soković, Marina
Marin, Petar
Grujić, Slavica
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.
Keywords:
Prunus spinosa; Anthocyanins; Antioxidant activity; Antitumor activity; Enzyme-inhibitory activity; Leaf extractsSource:
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2021, 49, 1, 12137-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.15835/nbha49112137
ISSN: 0255-965X
WoS: 000639294300001
Scopus: 2-s2.0-85104005337
URI
https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137https://radar.ibiss.bg.ac.rs/handle/123456789/4213