Nepetalactone-rich essential oil mitigates BASTA-induced ammonium toxicity in Arabidopsis thaliana L. by maintaining glutamine synthetase activity
2018
Authors:
Dmitrović, SlavicaDragićević, Milan
Savić, Jelena
Milutinović, Milica
Živković, Suzana
Maksimović, Vuk
Matekalo, Dragana
Perišić, Mirjana
Mišić, Danijela
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2018 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Phosphinothricin, active ingredient of the commercial herbicide BASTA, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation, which leads to the elevation of ammonium levels in plants and further to various physiological alternations, ammonium toxicity and lethality. Nepeta rtanjensis Diklić & Milojević essential oil (NrEO), rich in iridoid monoterpenoids nepetalactones, has been previously highlighted as a potential bioherbicide inducing oxidative stress in model plants. Interestingly, simultaneous foliar application of NrEO and BASTA, two agents showing differential mode of herbicidal action, suspends BASTA-induced toxicity in Arabidopsis thaliana plants by maintaining sub-toxic and/or sub-lethal ammonium concentration in tissues. NrEO effectively reduces BASTA efficacy, and the degree of antagonism between these agents escalates at increasing BASTA and NrEO levels. Regulation of GS activity, as influenced by BASTA, NrEO, and their joint action, partially occurs at transcriptional, posttranscriptional, and/or posttranslational levels, and is organ-specific. Furthermore, BASTA and NrEO interaction mitigates the effects of these agents, applied independently, on chlorophyll, soluble sugars and organic acids metabolism. Results suggest the existence of complex regulatory mechanisms determining antagonistic BASTA and NrEO interaction, and highlight the possible applications of the BASTA/NrEO mixture in agricultural practice. Furthermore, the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils, is suggested here as a environment-friendly approach for weed control.
Keywords:
BASTA; phosphinothricin; nepetalactone; antagonism; glutamine synthetase; ammonium toxicityFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-MESTD-Basic Research (BR or ON)-173040)
In:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade : Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 49.