Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes
2017
Authors:
Nenadić, MarijaLjaljević-Grbić, Milica
Stupar, Miloš
Vukojević, Jelena
Ćirić, Ana
Tešević, Vele
Vujisić, Ljubodrag
Todosijević, Marina
Vesović, Nikola
Živković, Nemanja
Ćurčić, Srećko
Document Type:
Article (Published version)
,
© 2017, Springer-Verlag Berlin Heidelberg
Metadata
Show full item recordAbstract:
The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus
punctatus from a cave in Southeastern Serbia against cavedwelling micromycetes, isolated from the same habitat, has
been investigated. Eleven collected samples were analyzed
and 32 isolates of cave-dwelling fungi were documented. A
total of 14 fungal species were identified as members of the
genera Aspergillus, Penicillium, Alternaria, Cladosporium,
Rhizopus, Trichoderma, Arthrinium, Aureobasidium,
Epicoccum, Talaromyces, and Fusarium. Five isolates were
selected for testing the antifungal activity of the pygidial gland
se cr etio n: Talaromyces d uclauxi, Aspergillus
brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and
Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and
minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent.
L. punctatus has developed a special mechanism of producing
specific compounds that act synergistically within the
secretion mixture, which are responsible for the antifungal
action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle
defense against pathogens, which could have an important
application in human medicine, in addition to the environmental impact, primarily.
Keywords:
Laemostenus punctatus; Troglophilic ground beetle; Pygidial gland secretion; Cave-dwelling micromycetes; Antifungal activitySource:
The Science of Nature, 2017, 104, 5-6, 52-Funding / projects:
- Ontogenetic characterization of phylogenetic biodiversity (RS-MESTD-Basic Research (BR or ON)-173038)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.1007/s00114-017-1474-4
ISSN: 0028-1042
PubMed: 28584964