Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp
2021
Authors:
Bertéli, Míria Benetati DelgadoBarros, Lillian
Reis, Filipa S.
Ferreira, Isabel C. F. R.
Glamočlija, Jasmina
Soković, Marina
Valle, Juliana Silveira
Linde, Giani Andrea
Ruiz, Suelen Pereira
Colauto, Nelson Barros
Document Type:
Article (Published version)
,
© 2021 by the The Royal Society of Chemistry.
Metadata
Show full item recordAbstract:
Lentinus crinitus (L.) Fr. (Basidiomycota: Polyporales) is a wild mushroom with several biotechnological
applications; however, there are few studies on its chemical composition and antimicrobial activity.
Therefore, this study aims to evaluate the chemical composition, cytotoxicity, and antimicrobial activity of
L. crinitus basidiocarp. For that, its nutritional value (AOAC procedures) and its composition in some
hydrophilic and lipophilic compounds (chromatographic techniques) were assessed. Moreover, the
potential hepatotoxic effects were evaluated using a primary cell culture obtained from porcine liver, and
its growth inhibitory capacity was also evaluated against four human tumour cell lines (spectrophotometric
assays). The antimicrobial activity was evaluated by microdilution against eight bacteria and fungi.
The basidiocarp has a high content of carbohydrates and, therefore, a relatively high energetic value. It is
also rich in soluble sugars, β-tocopherol, phenolic acids, mainly p-hydroxybenzoic acid, and organic
acids, mainly malic acid. L. crinitus did not show cytotoxicity in non-tumour cells, but it did not inhibit the
growth of human tumour cell lines either. The basidiocarp has a wide antimicrobial activity, inhibiting the
growth of different species of bacteria and fungi. It showed minimum bactericidal and fungicidal concentration values similar to or lower than those verified by commercial antibiotics or food additives used as preservatives. The antimicrobial activity was more evident against Listeria monocytogenes, Salmonella enterica, and Penicillium ochrochloron, followed by Aspergillus ochraceus and Trichoderma viride, when compared to the controls. The results obtained in this study showed that L. crinitus basidiocarp has great potential to be used by the industry without toxicity risks.
Keywords:
Lentinus crinitus; Mushroom; Chemical composition; Cytotoxicity; Antimicrobial activitySource:
Food & Function, 2021Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.1039/d1fo00656h
ISSN: 2042-6496
PubMed: 34109332