Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
2021
Authors:
Raspor, MartinMotyka, Václav
Kaleri, Abdul Rasheed
Ninković, Slavica
Tubić, Ljiljana
Cingel, Aleksandar
Ćosić, Tatjana
Document Type:
Article (Published version)
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Show full item recordAbstract:
De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
Keywords:
auxin; cytokinin; de novo shoot organogenesis; DNSO; gene regulatory network; hormone uptake; shoot regeneration; sucrose; transport; Auxin; Cytokinin; de novo shoot organogenesis; DNSO; Gene regulatory network; Hormone uptake; Shoot regeneration; Sucrose; TransportSource:
International Journal of Molecular Sciences, 2021, 22, 16, 8554-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/ijms22168554
ISSN: 1422-0067
PubMed: 34445260
WoS: 000690483600001
Scopus: 2-s2.0-85112657823
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IBISSAPA:
Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A., et al. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. International Journal of Molecular Sciences, 22(16), 8554.Vancouver:
Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. Int J Mol Sci. 2021;22(16):8554.Related items
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