Mucilaginous exudate from the apical root sections of Allium ascalonicum provokes autoinhibition of proliferation and subsequent bud regeneration
2018
Preuzimanje 🢃
Autori:
Zdravković-Korać, SnežanaĆalić, Dušica
Devrnja, Nina
Belić, Maja
Milojević, Jelena
Ostala autorstva
Uzelac, BrankaTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2018 by the Serbian Plant Physiology Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Mucilage secreted from in vitro-cultured organs has been frequently reported in numerous Allium species. Despite its adverse effect on cell proliferation and subsequent de novo regeneration,
this phenomenon has never been thoroughly studied. In Allium ascalonicum (shallot), only the
uppermost part of the root tip responds to a callus induction treatment by forming callus with
bud-forming capacity. The very same site of the root secretes mucilage during the course of its
cultivation on callus induction medium (CIM). To address this issue, mucilaginous exudate (10 μL),
collected from previously cultured root-tips of shallot, was applied directly to the root-tips isolated
from in vitro-cultured shallot plants. Untreated root-tips were used as a control. All explants were
cultivated on CIM containing 5 μM 2,4 D + 5 μM BA for 10 weeks, and then on regeneration induction medium supplemented with 5 μM BA for 4 weeks. A remarkable difference in cell proliferation
and subsequent bud regeneration between treated and control root-tips were observed. Calli
formed from treated root-tips were 3-fold lighter (53.7 mg vs. 169.9 mg) and regenerated buds
with lower frequency (14.64% vs. 89.2%) and smaller mean bud number per explant (0.1 vs. 15.4),
and these buds were shorter (0.65 mm vs. 16.4 mm) compared to the control. Root-tips isolated
from older roots secreted the mucilage at the higher frequency, formed smaller calli and regenerated fewer buds than those isolated from younger roots. Inhibition of cell proliferation indicates
the potential of this exudate in control of cell proliferation in the treatment of some diseases.
Ključne reči:
Allium ascalonicum; Bud regeneration; Mucilaginous exudate; Roots; ShallotFinansiranje / projekti:
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
- Grant No. 451- 03-1629/2017/2138
U:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 38.