Secondary somatic embryogenesis in Centaurium erythraea
2018
Autori:
Bogdanović, MilicaĆuković, Katarina
Dragićević, Milan
Simonović, Ana
Subotić, Angelina
Todorović, Slađana
Ostala autorstva
Uzelac, BrankaTip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2018 by the Serbian Plant Physiology Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Centaurium erythraea Rafn is a widespread medicinal plant from the Gentianaceae family.
Grown in vitro, centaury displays enviable developmental plasticity, often being capable of regenerating
the whole plant from root or shoot tissues through pathways of organogenesis and
somatic embryogenesis. Somatic embryogenesis (SE) is especially interesting for mass plant propagation,
production of virus-free material, synthetic seeds and cryopreservation, and is known to
produce less somaclonal variation than organogenesis. In Centaurium, both pathways have been
reported to occur both spontaneously and induced by plant growth regulators (2,4-D and CPPU).
It has been noted that subculturing of embryogenic callus for multiplication over extended period
of time results in the reduction of embryogenic potential. One way to overcome this problem is
initiation of secondary embryogenesis from primary cotyledonary embryos. Here we report successful
secondary embryogenesis in C. erythraea induced both directly and indirectly from cotyledonary
somatic embryos. Several combinations of different 2,4-D and CPPU concentrations were
tested for efficiency to initiate secondary embryogenesis in the dark. After four weeks, several
parameters were recorded: presence of callus and new cotyledonary embryos, number of cotyledonary
embryos per explant and whether new embryos were formed directly or indirectly. Secondary
embryos were capable of producing tertiary somatic embryos, effectively entering cyclic
SE. Secondary embryos, as well as primary, were able to germinate into plantlets by transferring
them on hormone-free medium.
Ključne reči:
Centaurium erythraea; somatic embryogenesis; 2,4-D; CPPUFinansiranje / projekti:
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)
- Razvoj i primena biotehnoloških postupaka u dobijanju zdravog sadnog materijala ukrasnih biljaka (RS-MESTD-Technological Development (TD or TR)-31019)
U:
- Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 34.