Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
2021
Authors:
Marinković, JelenaNikolić, Biljana
Marković, Tatjana
Radulović, Milena
Ilić, Jugoslav
Bošković, Marko
Ćirić, Ana
Marković, Dejan
Document Type:
Article (Published version)
,
© 2021 Future Medicine Ltd.
Metadata
Show full item recordAbstract:
The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus
oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and an tibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization
of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination
of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by
2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm
efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU count ing assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed
biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential.
Both substances induced some antioxidant activity. Conclusion: Results encourage further research with
the aim of application of the nanoemulsion in dental practice.
Keywords:
Antibiofilm activity; Cymbopogon citratus essential oil; Enterococcus faecalis; Infected teeth root canal; NanoemulsionSource:
Future Microbiology, 2021, 16, 12, 907-918Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-MESTD-inst-2020-200003)
DOI: 10.2217/fmb-2021-0081
ISSN: 1746-0913
PubMed: 34319146