Diploid vs. tetraploid Centaurium erythraea Rafn: a comparative study of regenerative in vitro potential and biosynthetic capacity
2019
Autori:
Filipović, BiljanaŠiler, Branislav
Nestorović Živković, Jasmina
Banjanac, Tijana
Skorić, Marijana
Božunović, Jelena
Mišić, Danijela
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The impact of ploidy level on both the regenerative potential under in vitro conditions and the production of major bioactive specialized metabolites, such as iridoids and xanthones, was examined in Centaurium erythraea Rafn. Shoot regeneration frequency was genotype dependent, but not affected by explant ploidy level. In most cases, the regenerated shoots of autotetraploid (4x) genotypes were more robust than diploid (2x) ones. Regeneration efficiency of root explants declined from the apical to the basal root segment. Shoot and root biomass production of two month-old plants was not significantly different between 2x and 4x genotypes. Both 4x and 2x genotypes were characterized by the predominance of secoiridoid glucoside gentiopicrin in shoots and roots, which is followed by swero-side and swertiamarin. Loganic acid, loganin and secologanin were much less abundant. Methylbel-lidifolin was the major xanthone in both shoots and roots. Diploid plants showed higher biosynthetic capacity for the production of secoiridoids and xanthones in both shoots and roots. Results highlight a higher potential of diploid C. erythraea genotypes for biotechnology-based sustainable production of secoiridoids in comparison to tetraploid genotypes.
Ključne reči:
Centaurium erythraea; Diploid; Autotetraploid; Morphogenesis in vitro; Secoiridoids; XanthonesIzvor:
Lekovite sirovine, 2019, 39, 52-59Finansiranje / projekti:
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)