The pygidial gland secretions of ground beetles (Insecta: Coleoptera: Carabidae): antimicrobial and antitumour activity of the natural products
2019
Autori:
Nenadić, MarijaSoković, Marina
Glamočlija, Jasmina
Ćirić, Ana
Tešević, Vele
Vujisić, Ljubodrag
Vesović, Nikola
Ćurčić, Srećko
Ostala autorstva
Soković, MarinaTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2019 Institute for Biological Research "Siniša Stanković", University of Belgrade
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In beetles, secretions are products of different exocrine glands, of which particular
emphasis is on pygidial glands, which are common for the suborder Adephaga.
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle
species was observed against human pathogens. A microdilution method was applied
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive
and gram-negative species, and eight fungal species). The secretions of tested ground
beetles have a certain level of antimicrobial activity, which differed between species,
which is caused by the difference in chemical composition of the mixtures. The highest
antibacterial effect was observed after treatment of most pathogens with secretion of
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic
strains was noticed after treatment of the secretion of Calosoma sycophanta, which
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C.
sycophanta each showed a strong antifungal effect compared to positive controls.
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor
cells by the secretion of adults of four ground beetle species was observed as well.
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested
secretions. All secretions have shown certain antiproliferative effect on the tested
cell lines, but statistically insignificant in most cases, which confirms the absence of
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L.
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value)
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity.
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground
beetles was demonstrated for the first time in this study.
U:
- Soković M, editor. Book of abstracts: The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; 2019. p 43.