Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields.
2021
Autori:
Ilijin, LarisaMrdaković, Marija
Todorović, Dajana
Vlahović, Milena
Grčić, Anja
Filipović, Aleksandra
Perić Mataruga, Vesna
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2021, Academia Brasileira de Ciencias
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch - off in the expression of constitutive and/or inducible HSP70 isoforms.
Ključne reči:
Acetylcholinesterase; HSP70 isoforms; Magnetic field; Relative growth rateIzvor:
Anais da Academia Brasileira de Ciencias, 2021, 93, 2, e20190118-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.1590/0001-3765202120190118
ISSN: 0001-3765
PubMed: 34105607
WoS: 000661511800001
Scopus: 2-s2.0-85107426627
URI
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=enhttps://radar.ibiss.bg.ac.rs/handle/123456789/4409