Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
2022
Аутори:
Živanović, JasminaJarić, Ivana
Ajdžanović, Vladimir
Miler, Marko
Stanković, Sanja
Milošević, Verica
Filipović, Branko
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2021 Elsevier GmbH
Метаподаци
Приказ свих података о документуАпстракт:
Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.
Кључне речи:
Andropause; Genistein; Kidney; Klotho; Mineral metabolismИзвор:
Annals of Anatomy - Anatomischer Anzeiger, 2022, 239, 151836-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.1016/j.aanat.2021.151836
ISSN: 0940-9602
PubMed: 34563672
WoS: 000707746900007
Scopus: 2-s2.0-85116546479
URI
https://linkinghub.elsevier.com/retrieve/pii/S094096022100162Xhttps://radar.ibiss.bg.ac.rs/handle/123456789/4499