Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
2021
Authors:
Trifunović-Momčilov, MilanaMotyka, Václav
Dobrev, Petre I.
Marković, Marija
Milošević, Snežana
Jevremović, Slađana
Dragićević, Ivana Č.
Subotić, Angelina
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
Keywords:
Centaury; Salinity; AtCKX genes; Cytokinin; Auxin; Stress hormonesSource:
Scientific Reports, 2021, 11, 21471-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Czech Science Foundation (project 19-12262S)
- Ministry of Education, Youth and Sports of the Czech Republic from European Regional Development Fund-Project "Centre for Experimental Plant Biology" (No. CZ.02.1.01/0.0/0.0/16_019/0000738)
DOI: 10.1038/s41598-021-00866-7
ISSN: 2045-2322
PubMed: 34728697