Hemijski sastav ekstrakata makromiceta i etarskih ulja divljeg origana i korijandera i njihov in vitro potencijal u tretmanu uzročnika hroničnog tonzilitisa
Chemical composition of macromycetes extracts, wild oregano and coriander essential oils and their in vitro potential in the treatment of chronic tonsillitis causative agents
Abstract:
For the investigation of natural products potential in the treatment of chronic tonsillitis, 7 species of macromycetes (Armillaria mellea, Lactarius piperatus, L. quietus, L. vellereus, Russula integra, R. nigricans i R. rosea) and 2 commercial essential oils (Origanum minutiflorum and Coriandrum sativum) were selected. Their chemical composition and biological activity were evaluated. Chemical analysis of extracts from fruiting bodies has shown they have a favorable nutritional profile (high content of carbohydrates and proteins, with low lipids content). The most common sugar was mannitol, while content of tocopherol isoforms was varied on the tested macromycetes species. The fatty acids analysis revealed high presence of linoleic, oleic and stearic acid, among the organic acids, malic acid was the most abundant, whereas p-hydroxybenzoic acid was the most predominant among phenolic acids. Regarding chemical analysis of essential oils, analysis showed that carvacrol was the most common compound in the wild growing oregano oil, while in case of coriander that was linalool. Antibacterial activity was evaluated on 16 different strains isolated from tissue samples of palatine tonsils and adenoids from patients diagnosed with chronic tonsillitis and identified. Macromycetes extracts showed moderate antibacterial activity, while tested essential oils exerted significantly better activity. Antioxidant activity determinated by TBARS method showed that the tested extracts, as well as wild oregano essential oil possess good in vitro antioxidant activity; while activity measured by OxHLIA method showed high antioxidant potential but only for L. vellereus, R. integra and R. nigricans samples. As for the cytotoxic potential towards tumor cell line NCI – H460 L.
Keywords:
Lactarius; Russula; essential oils; antibacterial activity; antioxidative activity; cytotoxic activity; chronic tonsillitisSource:
Faculty of Biology; University of Belgrade, 2021, 1-111Funding / projects:
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)