Strain differences in sterile inflammation and immune suppression induced by acute cadmium administration in rats depend on the affected activity and tissue
2015
Аутори:
Demenesku, JelenaMirkov, Ivana
Ninkov, Marina
Popov Aleksandrov, Aleksandra
Zolotarevski, Lidija
Subota, Vesna
Mileusnić, Dina
Kataranovski, Dragan
Kataranovski, Milena
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2015 by the European Federation of Immunological Societies
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: Conflicting data (suppression, augmentation, no effect) exist concerning cadmium (Cd) effects on immune system depending on activity and tissue examined. This study
investigates responses to acute Cd intoxication in three compartments (peripheral blood, spleen and lungs) in Dark Agouti (DA) and Albino Oxford (AO) rats, which are differently
susceptible to variety of stimuli.
Materials and Methods: Systemic (IL-6, TNF, acute phase proteins) and tissue responses [cell stress (metallothionein/MT gene expression), CD11b expression, and cytokine (IFN-γ,
IL-17, IL-10) production and mRNA expression] were measured following intraperitoneal (1 mg/kg) Cd administration.
Results: Cd induces systemic inflammatory response with similar intensity in both rat strains. Increase in Cd spleen content and MT expression evident in both strains (higher in DA
compared to AO rats) was followed by increase in neutrophil infiltration and CD11b expression (with same intensity). Although in both strains Cd caused decreased IFN-γ, unchanged
IL-17 and lower IL-10 responsiveness (compared to respective control), decrease of IFN-γ was more intense in DA compared to AO rats. In lungs of both strains increased Cd deposition
and MT expression (higher in AO) as well as neutrophil infiltration and CD11b expression (greater in DA) was observed. While decreased IFN-γ was noted in both strains, lower IL-17
and IL-10 (vs. controls) were evident in DA rats solely.
Conclusions: Acute Cd intoxication exerts strain-related effects (both inflammatory and immunosuppressive) depending on tissue and activity investigated, but the effects are more
pronounced in DA rats.
Кључне речи:
Dark Agouti (DA) and Albino Oxford (AO) rats; Sterile inflammation; Immune suppression; Acute cadmium administration; Spleen immune responses; Lung immune responsesУ:
- Abstract book: 4th European Congress of Immunology: ECI 2015; 2015 Sep 6-9, 2015; Vienna, Austria. European Federation of Immunological Societies; 2015. p. 353.