Transdermal toxicity of topically applied anticoagulant rodenticide warfarin in rats
2016
Authors:
Subota, VesnaMirkov, Ivana
Demenesku, Jelena
Popov Aleksandrov, Aleksandra
Ninkov, Marina
Mileusnić, Dina
Kataranovski, Dragan
Kataranovski, Milena
Document Type:
Article (Published version)
,
© 2015 Elsevier B.V.
Metadata
Show full item recordAbstract:
Occupational/accidental exposure data have showed hemorrhage as a result of transdermal exposure to
warfarin, however, other effects are not known. In the present study, the impact of epicutaneous application of 10 g or 100 g of warfarin (three times, once a day) on peripheral blood polymorphonuclear
(PMN) and mononuclear cells (PBMC) was examined in rats. Both doses resulted in prolongation of prothrombin time and changes in hematologic parameters. Increases in PMN intracellular myeloperoxidase
(MPO) activity were seen at higher warfarin dose and both doses resulted in higher percentages of granular CD11b+ cells. In contrast, a decrease in PMN TNF and IL-6 production (ELISA) and gene expression
(RT-PCR) was observed. Epicutaneous application of warfarin resulted in decreased numbers of PBMC,
higher numbers of mononuclear CD11b+ cells, but without effect on PMBC cytokine production. The data
obtained showed differential effects of transdermal exposure to warfarin depending on leukocyte type
and activity.
Keywords:
Rats; Rats Transdermal warfarin exposure; Peripheral blood leukocytesSource:
Environmental Toxicology and Pharmacology, 2016, 41, 232-240Funding / projects:
- Immunomodulatory effects of environmental xenobiotics and biotic factors on the populations of mouse-like rodents (RS-MESTD-Basic Research (BR or ON)-173039)
DOI: 10.1016/j.etap.2015.12.006
ISSN: 1382-6689
PubMed: 26742017