Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
2022
Autori:
Jurič, AndrejaBrčić Karačonji, Irena
Žunec, Suzana
Katić, Anja
Gašić, Uroš
Milojković Opsenica, Dušanka
Kopjar, Nevenka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2022 International Bee Research Association.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine in the Mediterranean area, especially in the treatment of wounds, burns and infections. In this study, we investigated the cyto-/genoprotective properties of STH at cell level following short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes (PBL) were selected as model cells. Phenolics in STH were determined using an ultra-high-performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with STH before and after irradiation was assessed by alkaline comet assay, while their viability was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress. Post-radiation treatment with STH was not as beneficial. Protective effects could be associated to the complex phytochemical profile of STH and the high content of HGA (306.8 mg/kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms, including intracellular antioxidative defence and DNA repair systems that helped pre-treated cells to counteract harmful exposures more efficiently.
Ključne reči:
Comet assay; UVB; Cyto/genotoxic effect; Polyphenols; Strawberry tree honeyIzvor:
Journal of Apicultural Research, 2022, 1-10Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS/ (-)
DOI: 10.1080/00218839.2022.2047421
ISSN: 0021-8839
WoS: 000771828400001
Scopus: 2-s2.0-85126854334
URI
https://www.tandfonline.com/doi/full/10.1080/00218839.2022.2047421http://radar.ibiss.bg.ac.rs/handle/123456789/4949