AMPK Activation Is Important for the Preservation of Insulin Sensitivity in Visceral, but Not in Subcutaneous Adipose Tissue of Postnatally Overfed Rat Model of Polycystic Ovary Syndrome
2022
Authors:
Mićić, BojanaTeofilović, Ana
Đorđević, Ana
Veličković, Nataša
Macut, Djuro
Vojnović-Milutinović, Danijela
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Polycystic ovary syndrome (PCOS) is a well-known reproductive syndrome usually associated
with obesity, insulin resistance, and hyperinsulinemia. Although the first signs of PCOS
begin early in adolescence, it is underexplored whether peripubertal obesity predisposes women to
PCOS metabolic disturbances. To highlight that, we examined the impact of postnatal overfeedinginduced
obesity, achieved by litter size reduction during the suckling period, on metabolic disturbances
associated with visceral and subcutaneous adipose tissue (VAT and SAT) function in the
5 -dihydrotestosterone (5 -DHT)-induced animal model of PCOS. We analyzed markers of insulin
signaling, lipid metabolism, and energy sensing in the VAT and SAT. Our results showed that postnatally
overfed DHT-treated Wistar rats had increased VAT mass with hypertrophic adipocytes,
together with hyperinsulinemia and increased HOMA index. In the VAT of these animals, insulin
signaling remained unchanged while lipogenic markers decreased, which was accompanied by
increased AMPK activation. In the SAT of the same animals, markers of lipogenesis and lipolysis
increased, while the activity of AMPK decreased. Taken together, obtained results showed that
postnatal overfeeding predisposes development of PCOS systemic insulin resistance, most likely as
a result of worsened metabolic function of SAT, while VAT preserved its tissue insulin sensitivity
through increased activity of AMPK.
Keywords:
polycystic ovary sindrome; obesity; visceral adipose tissue; subcutaneous adipose tissue; insulin resistance; 5a-dihydrotestosterone; early postnatal overfeeding;; lipogenesis; lipolysis; AMP-activated kinaseSource:
International Journal of Molecular Sciences, 2022, 23, 16, 8942-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/ijms23168942
ISSN: 1422-0067
PubMed: 36012206