Microbiological and histological analysis of tonsil tissue from patients diagnosed with chronic tonsilitis
2022
Authors:
Kostić, MarinaIvanov, Marija
Sanković Babić, Snežana
Tapavčević, Zvezdana
Radanović, Oliver
Ćirić, Ana
Soković, Marina
Document Type:
Conference object (Published version)
,
© 2022 by the Serbian Society of Microbiology
Metadata
Show full item recordAbstract:
If chronic tonsillitis (CT) is treated surgically, the long-term risks of infections and respiratory diseases are
higher, making of high importance to find an alternative for tonsillectomy. In order to isolate and identify
the causing agents of CT, a total of 79 postoperative palatine and adenoid tissue samples were obtained
from the ENT Clinic, KBC Zvezdara. A total of 82 bacterial species were isolated and identified by MALDI-TOF.
They belong to the genera Streptococcus, Staphylococcus, Micrococcus, Rothia, Enterobacter and Stenotrophomonas.
Analysis of the incidence of bacteria isolated from tissue samples showed the highest prevalence of Streptococcus
oralis (27/33%), followed by S. aureus (17/21%) and Streptococcus parasanquinis (16/20%). Histological analysis
of tissue samples was conducted in order to detect the presence of CT-causing bacteria. In addition to interfollicular
hyperplasia, colonies of species S. aureus and Stenotrophomonas maltophilia were detected in histological material.
The presented results confirm that the colonizers of tonsils tissues can be both G (+) and G (-) bacteria. The
presence of S. aureus in the tonsillar tissue even after the antibiotic therapy may be related to its ability to
form biofilm. Our study demonstrated that among 17 S. aureus isolates, 13 displays more pronounced biofilm
forming ability than the reference strain S. aureus ATCC 11632, and only 4 isolates display lower biofilm forming ability.
The presence of biofilm might be the reason for the recurrence of infection. Therefore, a searching for new
antibiofilm therapeutics in CT therapy is of great importance.
Keywords:
Tonsillitis; Biofilm; Staphylococcus aureusFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. Belgrade: Serbian Society of Microbiology; 2022. p. 853.
URI
https://www.femsbelgrade2022.org/abstract-bookhttp://radar.ibiss.bg.ac.rs/handle/123456789/5155