Fructose consumption affects glucocorticoid receptor signaling and increases lipogenesis in the liver of young female rats
2019
Autori:
Radovanović, MarinaKovačević, Sanja
Elaković, Ivana
Vojnović-Milutinović, Danijela
Matić, Gordana
Brkljačić, Jelena
Ostala autorstva
Spasojević, IvanTip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2019 by the Serbian Biochemical Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The effects of early-life fructose consumption and their relation to metabolic diseases risk
in adulthood are not yet elucidated. This study explored the direct effects of a diet regime
characterized by fructose enrichment on glucocorticoid receptor signaling in the liver of
female rats immediately after weaning. 21 day-old female Wistar rats were subjected to a 9
week-long diet regime involving standard chow in combination with either the 10%
fructose solution or tap water. Glucocorticoid receptor hormone binding parameters,
intracellular distribution of this molecule as well as the expression of its target genes
involved in lipid metabolism (most notably Lipin-1) and glucose metabolism (PEPCK),
were measured. An increase in the hepatic glucocorticoid receptor hormone binding
activity as well as an elevated nuclear translocation of the receptor, in concert with the
increased protein levels of Lipin-1 were observed after fructose enriched diet. This was
preceeded by a hepatic elevation in Glut-2 fructose transporter expression. Fructose-
enriched diet starting immediately after weaning enhanced hepatic glucocorticoid signaling
in young female rats and promoted lypogenesis as evidenced not only by the lipin-1
increase but also by FAS, ACC and SCREBP-1 expression elevations contributing to
hypertriglyceridemia and the expansion of the visceral adipose tissue 1, with no effect on
the hepatic gluconeogenesis. These results imply that while most parameters remained
within physiological reactivity, prolonged treatment might ultimately lead to more
pronounced metabolic disturbances.
Finansiranje / projekti:
- Uloga steroidnih hormona u neuroendokrinoj adaptaciji na stres i patofiziologiji metaboličkog sindroma - molekularni mehanizmi i kliničke implikacije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
U:
- Spasojević I, editor. The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia. Belgrade: Faculty of Chemistry; 2019. p. 141.