Beyond Traditional Use of Alchemilla vulgaris: Genoprotective and Antitumor Activity In Vitro
2022
Authors:
Jelača, SanjaDajić-Stevanović, Zora
Vuković, Nenad
Kolašinac, Stefan
Trendafilova, Antoaneta
Nedialkov, Paraskev
Stanković, Miroslava
Tanić, Nasta
Tanić, Nikola
Acović, Aleksandar
Mijatović, Sanja
Maksimović-Ivanić, Danijela
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Alchemilla vulgaris L. (lady’s mantle) was used for centuries in Europe and Balkan countries for treatments of numerous conditions and diseases of the reproductive system, yet some of the biological activities of lady’s mantle have been poorly studied and neglected. The present study aimed to estimate the potential of A. vulgaris ethanolic extract from Southeast Serbia to prevent and suppress tumor development in vitro, validated by antioxidant, genoprotective, and cytotoxic properties. A total of 45 compounds were detected by UHPLC–HRMS analysis in A. vulgaris ethanolic extract. Measurement of antioxidant activity revealed the significant potential of the tested extract to scavenge free radicals. In addition, the analysis of micronuclei showed an in vitro protective effect on chromosome aberrations in peripheral human lymphocytes. A. vulgaris extract strongly suppressed the growth of human cell lines derived from different types of tumors (MCF-7, A375, A549, and HCT116). The observed antitumor effect is realized through the blockade of cell division, caspase-dependent apoptosis, and autophagic cell death. Our study has shown that Alchemilla vulgaris L. is a valuable source of bioactive compounds able to protect the subcellular structure from damage, thus preventing tumorigenesis as well as suppressing tumor cell growth.
Keywords:
Alchemilla vulgaris L.; antitumor action; antioxidative activity; genoprotective effectSource:
Molecules, 2022, 27, 23, 8113-Funding / projects:
- joint funding of Development and Research Projects of the Republic of Serbia and the People’s Republic of China (grant number 06/2018)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/molecules27238113
ISSN: 1420-3049
PubMed: 36500205