Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
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2023
Authors:
Trifunović-Momčilov, Milana![](/themes/MirageIBISS/images/orcid.png)
Stamenković, Nikola
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Đurić, Marija
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Milošević, Snežana
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Marković, Marija
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Giba, Zlatko
Subotić, Angelina
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Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
Keywords:
common centaury; salinity stress; Oxidative stress; antioxidative protection; sodium nitroprussideSource:
Life, 2023, 13, 1, 154-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/life13010154
ISSN: 2075-1729
PubMed: 36676103