Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
2023
Authors:
Mileski, Ksenija S.Ćirić, Ana
Gašić, Uroš
Žarković, Lazar D.
Krivošej, Zoran Đ.
Džamić, Ana M.
Document Type:
Article (Published version)
,
© 2022 ACG Publications.
Metadata
Show full item recordAbstract:
The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
Keywords:
Laserpitium siler; phenolics; LC-MS; antioxidant; antimicrobial; Pseudomonas aeruginosa; BSA; enzyme inhibitory activitySource:
Records of Natural Products, 2023, 17, 3, 453-475Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
DOI: 10.25135/rnp.364.2207.2528
ISSN: 1307-6167
WoS: 000891126100001
Scopus: 2-s2.0-85152695702
URI
https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdfhttp://radar.ibiss.bg.ac.rs/handle/123456789/5345