Naïve astrocytes react to CNS-infiltrated immune cells
2022
Authors:
Milićević, KatarinaLazarević, Milica
Momčilović, Miljana
Todorović, Nataša
Petković, Branka
Stojadinović, Gordana
Nikolić, Ljiljana
Contributors
Čupić, ŽeljkoAnić, Slobodan
Document Type:
Conference object (Published version)
,
© 2022 by the Society of Physical Chemists of Serbia
Metadata
Show full item recordAbstract:
Multiple sclerosis (MS) is an inflammatory disorder of the central nervous system (CNS)
characterized by infiltration of lymphocytes that leads to myelin damage and neurodegeneration.
The complex interaction between CNS-infiltrating immune cells (CNS-IIC) and astrocytes is an
important contributor to the disease progression. Here, we investigate how naïve astrocytes respond
to autoreactive immune cells present in the CNS at different stages of the disease. For this purpose,
CNS-IICs were isolated from the spinal cords of rats with experimental autoimmune
encephalomyelitis at onset, late-onset and the peak of the disease. Naïve astrocytes, isolated from
the spinal cords of wild-type rat pups, responded to brief bath application of CNS-IIC by robust
elevation of intracellular Ca2+ independently of the disease stage. Our data suggest that direct
contact between astrocytes and CNS-IICs induces Ca2+ changes in astrocytes and points to the new
aspect of cell-cell interactions in the propagation of neuroinflammatory response in CNS
autoimmunity.
Keywords:
astrocyte; immune cell; CNS autoimmune diseaseFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
In:
- Čupić Ž, Anić S, editors. Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. Belgrade: Society of Physical Chemists of Serbia; 2022. p. 267-70.