Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response
2007
Autori:
Mihailović, MirjanaDinić, Svetlana
Bogojević, Desanka
Ivanović-Matić, Svetlana
Uskoković, Aleksandra
Arambašić Jovanović, Jelena
Grigorov, Ilijana
Grdović, Nevena
Vidaković, Melita
Martinović, Vesna
Petrović, Miodrag
Poznanović, Goran
Ostala autorstva
Węgrzyn, GrzegorzTip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual’s
life and during the acute-phase (AP) response. Details of the participation of the STAT
family of transcription factors in its control remain incompletely understood. Here we examined
the involvement of STAT5b in MG gene expression during development and the AP response.
Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal
development, whereas in the adult it decreased. Stimulation of MG expression during the
AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization
revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in
the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene
(–825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding.
The highest concentration of the promoter-binding form of STAT5b was observed in the fetus.
As postnatal development progressed, the level of promoter-bound STAT5b decreased and in
the adult liver it was the lowest. Stimulation of MG gene expression during the AP response
in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the
MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine
phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine
phosphorylated species were completely absent. We conclude that developmental-stage differences
in the mechanisms that determine STAT5b nuclear localization contribute to its activity
in vivo.
Ključne reči:
α2-macroglobulin; STAT5b; rat liver development; phosphorylation; nuclear extract; nuclear matrixIzvor:
Acta Biochimica Polonica, 2007, 54, 2, 331-340Finansiranje / projekti:
- Akutni i hronični stres: mehanizmi regulacije homeostaze u akutnoj radijacionoj bolesti i dijabetesu (RS-MESTD-MPN2006-2010-143002)
DOI: 10.18388/abp.2007_3254
ISSN: 0001-527X
PubMed: 17565389