Metabolomic evaluation of five Nepeta species (fam. Lamiaceae) using liquid chromatography with multistage mass spectrometry
2022
Authors:
Gašić, UrošNestorović Živković, Jasmina
Dmitrović, Slavica
Aničić, Neda
Milutinović, Milica
Skorić, Marijana
Božunović, Jelena
Petrović, Luka
Šiler, Branislav
Filipović, Biljana
Banjanac, Tijana
Matekalo, Dragana
Todorović, Miloš
Lukić, Tamara
Dragićević, Milan
Mišić, Danijela
Contributors
Baebler, ŠpelaDermastia, Marina
Grebenc, Tine
Praprotnik, Eva
Razinger, Jaka
Urbanek Krajnc, Andreja
Document Type:
Conference object (Published version)
,
© 2022 by the Slovenian Society of Plant Biology
Metadata
Show full item recordAbstract:
The aim of this study was to determine qualitative composition of methanol-soluble metabolites in leaves
from in vitro-grown plants of phylogenetically distinct Nepeta species producing iridoids (N. sibirica L., N.
spicata Benth., N. argolica Bory & Chaub., and N. rtanjensis Diklić & Milojević) and iridoid-lacking N. nervosa
Royle ex Benth., adopting UHPLC-LTQ OrbiTrap MS technique. Totally, 52 compounds were identified
based on their monoisotopic masses, MSn fragmentation, and previously reported MS data and they were
clustered into four major groups: phenolic acid derivatives (23 compounds), flavonoids (10 compounds),
iridoids (13 compounds), and 6 compounds belonging to other classes. Both phenolic and iridoid profiles
of the tested species were found to be very diverse regarding both qualitative content of compounds and
their relative representation in extracts. Rosmarinic, 5-O-cafeoylquinic, and caffeic acids were found to be
the dominant phenolics in all analyzed species, especially in N. argolica, N. rtanjensis, and N. nervosa.
Iridoids were present in the form of glucosides and the most abundant compounds from this group were
epideoxyloganic acid, geniposide, and aucubin. Although iridoids were not detected in N. nervosa extracts,
this species was revealed as a rich source of phenolic acids and flavonoids. Conversely, N. spicata was found
to produce iridoids but negligible amounts of phenolic compounds. Extracts of two closely related endemic
species originating from the Balkan Peninsula, N. argolica and N. rtanjensis, were very similar in both
phenolic and iridoid profiles. The UHPLC-LTQ OrbiTrap MS method, in combination with chemometrics
data processing, was proved to be simple, rapid, and accurate strategy for unambiguous taxonomic
determination and chemical differentiation of Nepeta species.
Funding / projects:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
In:
- Baebler Š, Dermastia M, Grebenc T, Praprotnik E, Razinger J, Urbanek KrajncA , editors. Book of abstracts: Plants in Changing Environment: International conference of the Slovenian Society of Plant Biology; 2022 Sep 15-16; Ljubljana, Slovenia. Ljubljana: Slovenian Society of Plant Biology; 2022. p. 35.