UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.
2022
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Authors:
Marković, TijanaAničić, Neda
Marković Hadžić, Stefan
Božunović, Jelena
Dmitrović, Slavica
Nestorović Živković, Jasmina
Gašić, Uroš
Milutinović, Milica
Petrović, Luka
Mišić, Danijela
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
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Show full item recordAbstract:
Because of their sessile lifestyle, plants have been constantly facing ultraviolet (UV-B) radiation,
a type of abiotic stress which affects their overall growth, development, physiology, and metabolism. Qualitative and quantitative content of specialized metabolites, chemical compounds that
allow plants to adapt to specific conditions of the environment, is also affected. Nepeta nuda L.
(fam. Lamiaceae) is characterized by a significant amount of iridoids, monoterpenoid compounds
with prominent pharmacological effects. The aim of this study was to detect changes in the content of major iridoids (1,5,9-epideoxyloganic acid (1,5,9-eDLA), nepetalactone (NL), and dehydronepetalactone (DNL)) in N. nuda plants after exposure to UV-B radiation (during 30 and 60 min),
in parallel with expression analysis of genes participating in the biosynthetic pathway of iridoids
(NnGAPDH, NnGPPS, NnGES, NnG8O, Nn8HGO, NnIS, NnNEPS). After both UV-B treatments, the gene
GPPS showed the highest increase in gene expression, relative to control plants, while the gene
G8O showed the highest decrease in expression level. At the same time, UHPLC/DAD/(±)HESI-MS2
analysis revealed an increase in the concentration of 1,5,9-eDLA and DNL following the exposure
of plants to UV-B radiation for 60 minutes. In the case of a 30-minute treatment, there was an
increase in the concentration of eDLA, while the concentration of DNL was reduced, relative to
non-treated plants. The present study brings novel insights into the molecular background of
iridoid biosynthesis in UV-B stressed plants, which could help in optimizing the elicitation procedures and thus accumulation of bioactive compounds in N. nuda.
Keywords:
Nepeta nuda; UV-B radiation; nepetalactone; epideoxyloganic acid; dehydronepetalactoneFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 85.