Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?
2022
Autori:
Đorđević, JelenaOalđe Pavlović, Mariana
Jovanović Marić, Jovana
Kolarević, Stoimir
Duletić-Laušević, Sonja
Vuković-Gačić, Branka
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2022 by the Serbian Society of Microbiology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary
approach for health maintenance. In addition to their healing properties and application in folk medicine,
Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species
on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations
(double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%),
and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.082
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus
officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the
biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract
exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations
except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested
concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the
biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on
the degradation of the previously formed biofilm of P. aeruginosa PAO1.
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
U:
- Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. Belgrade: Serbian Society of Microbiology; 2022. p. 801-2.