Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro
2019
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Аутори:
Mićanović, DragicaSaksida, Tamara
Koprivica, Ivan
Vujičić, Milica
Despotović, Sanja
Šavikin, Katarina
Janković, Teodora
Stojanović, Ivana D.
Остала ауторства
Spasojević, IvanТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Serbian Biochemical Society
Метаподаци
Приказ свих података о документуАпстракт:
Chokeberry (Aronia melanocarpa) is known for its strong anti-oxidant properties. Antiinflammatory,
anti-hypertensive and anti-diabetogenic activities of orally consumed
chokeberry extracts have also been reported. The effects of chokeberry extract on the
immune response parameters have been only sporadically assessed. Therefore, the aim of
our study was to investigate the effects of orally consumed chokeberry extract on the
immune response in vivo and in vitro in healthy and in diabetic C57BL/6 mice, in which
diabetes was induced by multiple low doses of streptozotocin (MLDS). Chokeberry extract
administered to healthy mice (50 mg/kg body weight) exerted immunomodulatory effects
as evidenced by decreased proportion of F4/80+ macrophages, CD11c+ dendritic cells,
CD4+ T helper cells, CD8+ T cytotoxic lymphocytes and CD4+CD25- activated T
lymphocytes within the gut-associated lymphoid tissue. Surprisingly, oral consumption of
chokeberry extract in doses of either 200 mg/kg bw or 50 mg/kg bw in diabetic mice
resulted in the increase of blood glucose levels. In an attempt to decipher the underlying
mechanisms of chokeberry extract effects in the context of autoimmune/inflammatory
disease, we have evaluated its effects in vitro on purified immune cells. Seemingly, the
chokeberry extract exerted pro-inflammatory effects in vitro through the up-regulation of
nitric oxide and IL-1β production in macrophages and dendritic cells, increased
macrophage CD86-related activation and promotion of type 1 T helper cells (IFN-γ+)
differentiation. In addition, an increased proportion of CD4+, CD8+ and B lymphocytes
within the spleen was observed. Collectively, the obtained results imply that our particular
chokeberry extract displays pro-inflammatory characteristics and that care should be taken
when chokeberry is to be included in the human diet.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Spasojević I, editor. The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia. Belgrade: Faculty of Chemistry; 2019. p. 96.