Effects of cuprizone-induced demyelination on autophagy markers in different neural structures with the evaluation of behavior in rats
2023
Autori:
Zeković, JankoVidičević Novaković, Sašenka
Tasić, Jelena
Stanojević, Željka
Milovanović, Andona
Tomonjić, Nina
Petričević, Saša
Zogović, Nevena
Tovilović-Kovačević, Gordana
Trajković, Vladimir
Isaković, Aleksandra
Ostala autorstva
Kanazir, SelmaSavić, Danijela
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2023 by Serbian Neuroscience Society and associates
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Introduction: Cuprizone is a copper chelating agent rensposible for toxic
demyelination. Although its mechanism of demyelination is not fully elucidated, its
toxicity is thought to result, at least partially, from induced autophagy.
The Aim: To examine the effect of cuprizone on autophagy markers in different
neural structures in rats with the assessment of behavior in the open field test.
Material and Methods: This study was performed on 16 female DA rats, 6 weeks
old.The control group (n=8) did not receive cuprizone, while the experimental group
(n=8) received food with 0.6% cuprizone content during 7 weeks. Afterwards,
locomotion and anxiety-like behavior were assessed in the open field test. Imunoblot
technique was performed on: cortex, corpus callosum, cerebellum and spinal cord, in
order to examine the markers of autophagy signaling pathway: pUlk, p62, Beclin1,
LC3II, pAMPK, pmTOR and pRaptor.
Results: Cuprizone-fed animals had a reduced locomotor activity and exhibited an
anxiety-like behavior. In the cortex, p62 and pAMPK were decreased, while pmTOR
tended to be lower. In the corpus callosum, pULK was increased and pmTOR was
decreased. LC3II tended to be increased, while pAMPK tended to be decreased. In the
cerebellum, pAMPK was reduced, while pmTOR was increased. In the spinal cord,
Beclin1 and pRaptor were decreased, while pmTOR, pAMPK, LC3II, p62 tended to
be decreased.
Conclusion: Long-term use of cuprizone leads to impaired locomotor activity and
anxiety-like behavior. Cuprizone-induced damage is most likely due to AMPK-
independent autophagy in the cortex and corpus callosum, while autophagy is
probably inhibited in the cerebellum and spinal cord.
Ključne reči:
cuprizone; demyelination; autophagy; behaviorU:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 80.