Effects of different anesthetics on hippocampal and reticulothalamic GABAergic parvalbumin-expressing interneurons
2023
Autori:
Novaković, AndreaRadovanović, Ljiljana
Petrović, Jelena
Šaponjić, Jasna
Ostala autorstva
Kanazir, SelmaSavić, Danijela
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2023 by Serbian Neuroscience Society and associates
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
We examined the effects of ketamine/diazepam and propofol anesthesia on
hippocampal and reticulo-thalamic (RT) GABAergic parvalbumin (PV)-expressing
interneurons in the rat.
A total of 20 adult male Wistar rats were divided into two experimental groups - half
were anesthetized with ketamine/diazepam (100 mg/kg, Zoletil® 50), and the other
half received propofol anesthesia (100 mg/kg; Propofol Lipuro 2% (20mg/ml). We
performed immunohistochemistry protocols for PV and postsynaptic density protein
95 (PSD-95) staining on free-floating 40-µm brain slices. We used PSD-95 as an
excitatory synaptic marker to test local excitation changes along with changes in PV
expression.
Our results show significant suppression of GABAergic PV-expressing interneurons
during ketamine/diazepam anesthesia compared with propofol anesthesia, in the
dentate gyrus and CA3 regions of the hippocampus (z ≥ -4.16, p ≤ 10-3), and in the
RT. Moreover, this suppression resulted in an increase in PSD-95 expression only in
the hippocampus of rats anesthetized with ketamine/diazepam. Topographically
distinct effects of propofol anesthesia were not detected.
The observed imbalance between excitation and inhibition at the level of the
hippocampus during ketamine/diazepam anesthesia could be a consequence of lower
interneuronal GABA activity. Conversely, the topographically uniform expression of
PSD-95 during propofol anesthesia together with higher expression of GABAergic
interneurons could possibly indicate a stronger effect of GABA-mediated inhibition in
the hippocampus compared with ketamine/diazepam anesthesia.
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
U:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 52.