Anti-aging properties of protein persulfidation
2018
Preuzimanje 🢃
Autori:
Živanović, JasminaKouroussis, Emilia
Miler, Marko
Schott-Roux, Sonia
Jarić, Ivana
Jakovljević, Vladimir
Milošević, Verica
Filipović R., Miloš
Ostala autorstva
Đurić, DraganJakovljević, Vladimir
Živković, Vladimir
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2018 by the Serbian Physiological Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
An oxidative-posttranslational modification of thiols called protein persulfidation
(RSSH) represents the underlying mechanism of biological actions ascribed to new gasotransmitter hydrogen sulfide (H2S). We developed a new dimedone based method for detection of protein persulfidation and its selectivity was tested and confirmed in various experimental models (D. melanogaster, C. elegans, M. musculus. human cell lines and samples). Obtained results demonstrated that persulfidation represents an evolutionary conserved modification that correlates to the H2S production capacity and is accompanied with numerous physiological implications. One of such general observations is that persulfidation represents protective mechanism for the reduction of sulfenic acids under conditions of oxidative stress. When C. elegans mutants for CSE and MST were exposed to different oxidative stress inducers they showed enhanced sensitivity, while pretreatment with H2S donors rescued them. Additionally, we observed a strong correlation between protein persulfidation levels and development and aging in different experimental models. Fluctuations in the level of persultides were observed during development of C. elegans with prominent increase in L3 larval stage. Our data showed that after strong peak in L3, persulfide levels decreases during life in C. elegans. Decline of persulfide levels in the adult worms of different age was also observed. Similar effect was discovered in the brains of rats of different age. Immunobistological analysis of brain sections for H2S producing enzymes revealed the decrease in intensity of immunopositivity during aging process. Our results strongly propose protein persullidation represents a rescue loop from cysteine hyper-oxidation and subsequent loss of protein function. This general mechanism represents a promising tool in overcoming the consequences of increased oxidative stress during aging process.
Finansiranje / projekti:
- Odgovor neuroendokrinog sistema pacova na odabrane biljne ekstrakte, fitoestrogene, steroidne i peptidne hormone (RS-MESTD-Basic Research (BR or ON)-173009)
U:
- Đurić DM, Jakovljević V, Živković V, editors. Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. Belgrade: Serbian Physiological Society; 2018. p. 143.